Coding

Part:BBa_K3117012:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)


pelB signal sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pelB was fused to the 5' end of the constructs for targeting the recombinant proteins to the periplasm.


Source

pelB originates from genomic DNA.

References

1. Singh, Pranveer; Sharma, Likhesh; Kulothungan, S. Rajendra; Adkar, Bharat V.; Prajapati, Ravindra Singh; Ali, P. Shaik Syed et al. (2013): Effect of signal peptide on stability and folding of Escherichia coli thioredoxin. In: PloS one 8 (5), e63442. DOI: 10.1371/journal.pone.0063442.

2. Schlegel, Susan; Rujas, Edurne; Ytterberg, Anders Jimmy; Zubarev, Roman A.; Luirink, Joen; Gier, Jan-Willem de (2013): Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels. In: Microbial cell factories 12, S. 24. DOI: 10.1186/1475-2859-12-24.